In reverse chronological order I have been using Empower 2, Empower, Millennium (all those for both GC and HPLC), Water's 860 Vax based Expert Ease fro HPLC. I understand the structure, and being used to thinking in terms of relational databases find, it logical, easy to use, extremely powerful AND extremely flexible. You rarely have to deal with datafiles with user unfriendly names and paths. I can review and process data anyway way I want very simply.

And everything is linked together conveniently but transparently. I only have to worry about high level stuff. I just got a new job where they don't like Water's and they just bought a new Agilent HPLC with Chemstation. First doesn't Agilent know that the Multiple Document Interface (MDI) UI on Windows fell out of favor long ago????

That alone is causing me grief! Outside of actually doing an injection (I REALLY don't need cartoons for that.) I'm find this software amazing convoluted, unintuitive and limited in it's method part organization and data review capabilities!

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Separate on-line and off line apps???!!! Maybe it's just my unfamiliarity with Chemstation.

Agilent Chemstation Software Registration Code G1701BA GCMS MSD B.01.00 See more like this Agilent G1956 LC / ChemStation Installation, Guide & Owners Manual Pre-Owned.

But in general i'm pretty good with software (both using and writing) and i'm ready to start pulling out my hair. So can I ask questions about it here? First question. Is it possible top overlay two chromatograms in review mode from different acquisition sequences? In Empower i would just select any number of channels or results regardless of when they were acquired or processed and review them. If I want I can then easily look at some or all of the injection as overlays or stacked plots.

I've figured out (more or less) how to do that for injections in a single acquired sequence in ChemStation. But can I do it across sequences?

Agilent Chemstation Software Manual

Thanks, - Karen. Karen01 wrote: First question. Is it possible top overlay two chromatograms in review mode from different acquisition sequences? Under view, make sure you are in 'Full Menu. Then in Data Analysis, load the first data signal from its data subdirectory (in Sequence Parameters or Sample data name each day's data in a separate 1 Data subdirectory, such as 112310 for today's date).

Then select overlay (the next choice down from load signal), find the data directory where that data lies, and load that to overlay. Under Graphics, first selection, you can choose overlay or separated, and whether to zoom independently. You could also copy chromatogram xxx.D directories to a different data directory if that is easier.

The default ChemStation naming sequence is 001-0101.D where the first three characters are the vial number, the first two after the dash are the LINE in the Sequence Table, and the last two are which injection on that line. Under view, make sure you are in 'Full Menu. Then in Data Analysis, load the first data signal from its data subdirectory (in Sequence Parameters or Sample data name each day's data in a separate 1 Data subdirectory, such as 112310 for today's date).

Then select overlay (the next choice down from load signal), find the data directory where that data lies, and load that to overlay. Under Graphics, first selection, you can choose overlay or separated, and whether to zoom independently. Thanks I appreciate it! I'll try that tomorrow I would much rather see sample names. Having to do it using such file names makes it hard to quickly find the data you want to overlay. That's they type of thing that drives me crazy!!!! Why in this day and age should a chromatographer have to deal with cryptic files names instead of easily readable and meaningful sample names and and other info?